Genome editing by natural and engineered CRISPR-associated nucleases
Publication date
2018-07-01
Authors
Wu, Wen Y.
Lebbink, Joyce H.G.
Kanaar, Roland
Geijsen, Niels
Van Der Oost, John
Editors
Advisors
Supervisors
Document Type
Article
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taverne
Abstract
Over the last decade, research on distinct types of CRISPR systems has revealed many structural and functional variations. Recently, several novel types of single-polypeptide CRISPR-associated systems have been discovered including Cas12a/Cpf1 and Cas13a/C2c2. Despite distant similarities to Cas9, these additional systems have unique structural and functional features, providing new opportunities for genome editing applications. Here, relevant fundamental features of natural and engineered CRISPR-Cas variants are compared. Moreover, practical matters are discussed that are essential for dedicated genome editing applications, including nuclease regulation and delivery, target specificity, as well as host repair diversity.
Keywords
Taverne, Molecular Biology, Cell Biology
Citation
Wu, W Y, Lebbink, J H G, Kanaar, R, Geijsen, N & Van Der Oost, J 2018, 'Genome editing by natural and engineered CRISPR-associated nucleases', Nature Chemical Biology, vol. 14, no. 7, pp. 642-651. https://doi.org/10.1038/s41589-018-0080-x