Nanobody technology in thrombosis and haemostasis research

Abstract

Single domain antibodies (nanobodies) derived from camelids are interesting tools to use in coagulation research. They are characterized by a small size an ability to recognize hidden or specific molecular epitopes. These attributes are complemented by their high-binding affinity. This makes them perfectly suitable for studying activated coagulation factors, where there is a need for a subtle distinction in molecular conformation. In this thesis, activated coagulation factors have been studies with the aid of nanobodies. Novel nanobodies or assays for factor VIIa (FVIIa), active Von Willebrand Factor (active VWF) and platelet membrane receptor LRP8 are described. Furthermore, it is shown that FVIIa and active VWF play a role in the pathophysioly of critically ill patients and might be used as prognostic markers.