Profiling of volatile organic compounds produced by clinical Aspergillus isolates using gas chromatography-mass spectrometry
Publication date
2018-02
Authors
Gerritsen, M G
Brinkman, P
Escobar Salazar, N.
Bos, L D
de Heer, K
Meijer, Martin
Janssen, H-G
de Cock, Hans
Wösten, Han
Visser, C.E.
Editors
Advisors
Supervisors
Document Type
Article
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taverne
Abstract
Volatile organic compounds (VOCs) in exhaled breath may identify the presence of invasive pulmonary aspergillosis. We aimed to detect VOC profiles emitted by in vitro cultured, clinical Aspergillus isolates using gas chromatography-mass spectrometry (GC-MS). Three clinical Aspergillus isolates and a reference strain were cultured while conidiation was prevented. Headspace samples were analyzed using a standardized method. Breath samples of patients from which the cultures were obtained were checked for the presence of the VOCs found in vitro. Each Aspergillus isolate produced a distinct VOC profile. These profiles could not be confirmed in exhaled breath in vivo.
Keywords
gas chromatography-mass spectrometry, Aspergillus, headspace analysis, exhaled breath analysis, invasive pulmonary aspergillosis, Taverne
Citation
Gerritsen, M G, Brinkman, P, Escobar Salazar, N, Bos, L D, de Heer, K, Meijer, M, Janssen, H-G, de Cock, H, Wösten, H A B, Visser, C E, van Oers, M H J & Sterk, P J 2018, 'Profiling of volatile organic compounds produced by clinical Aspergillus isolates using gas chromatography-mass spectrometry', Medical Mycology, vol. 56, no. 2, pp. 253–256. https://doi.org/10.1093/mmy/myx035