Isofocusing and immunoelectrophoretic studies of soluble eye lens proteins from regenerated and normally developed Xenopus laevis

Abstract

Soluble eye lens proteins from regenerated and normally developed 6-month-old Xenopus laevis ap/ap-mutant froglets were compared by thin-layer isoelectric focusing and microimmunoelectrophoresis. Larvae belonging to the same age group were reared through metamorphosis after the removal of the left lens at different larval stages as explained in the text. The results show that regenerating and normally developing lenses of similar ages have identical profiles of α-, β- and γ-crystallins and also identical antigenic determinants suggesting that gene expression for lens crystallins follows the same pathway in a regenerating lens as in a normally developing lens.