Parinaric acid as a sensitive fluorescent probe for the determination of lipid peroxidation
Publication date
1987-09-25
Authors
Kuypers, F.A.
Berg, J.J.M. van den
Schalkwijk, C.
Roelofsen, B.
Op den Kamp, J.A.F.
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Article
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Abstract
The decrease in fluorescence of conjugated polyenic acyl chains is used as a sensitive assay for lipid peroxidation. The fatty acid cis-trans-trans-cis-9,11,13,15-octadecatetraenoic acid (cis-parinaric acid) is introduced into liposomal membranes as free fatty acid or, by using the PC specific transfer protein from bovine liver, as 1-palmitoyl-2-cis-parinaroyl-sn-glycero-3-phosphocholine. The peroxidation process as monitored by the decrease in fluorescence intensity is compared with other peroxidation assay systems. Applications of the new assay system are discussed.
Keywords
lipid peroxidation, parinaric acid, fluorescent probe, phosphatidylcholine, (Bovine liver)