A single point mutation creating a furin cleavage site in the spike protein renders porcine epidemic diarrhea coronavirus trypsin-independent for cell entry and fusion
Publication date
2015
Authors
Li, Wentao
Wicht, Oliver
van Kuppeveld, Frank J M
He, Qigai
Rottier, P.J.M.
Bosch, Berend Jan
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Supervisors
Document Type
Article
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Abstract
The emerging porcine epidemic diarrhea virus (PEDV) requires trypsin supplementation to activate its S protein for membrane fusion and virus propagation in cell culture. By substitution of a single amino acid in the S protein we created a recombinant PEDV (PEDV-S(FCS)) with an artificial furin protease cleavage site N-terminal of the putative fusion peptide. PEDV-S(FCS) exhibited trypsin-independent cell-cell fusion and was able to replicate in culture cells independent of trypsin, though to low titer.
Keywords
coronavirus, Coronacrisis-Taverne, SDG 3 - Good Health and Well-being
Citation
Li, W, Wicht, O, van Kuppeveld, F J M, He, Q, Rottier, P J M & Bosch, B-J 2015, 'A single point mutation creating a furin cleavage site in the spike protein renders porcine epidemic diarrhea coronavirus trypsin-independent for cell entry and fusion', Journal of Virology, vol. 89, no. 15, pp. 8077-8081. https://doi.org/10.1128/JVI.00356-15