Generation of induced pluripotent stem cells in defined three-dimensional hydrogels
Publication date
2017-09-05
Authors
Caiazzo, Massimiliano
Tabata, Yoji
Lutolf, Matthias P
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Supervisors
Document Type
Part of book
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taverne
Abstract
Since the groundbreaking discovery of induced pluripotent stem cells (iPSCs) many research groups have attempted to improve the efficiency of the classical cell reprogramming process. Surprisingly, the contribution of the three-dimensional (3D) microenvironment to iPSC generation has been largely overlooked. Here we describe a protocol for the generation of iPSCs in defined poly(ethylene glycol) (PEG)-based hydrogels that, besides allowing higher reprogramming efficiency, are also a powerful tool to study the influence of biophysical parameters on iPSC generation.
Keywords
3D culture, Bioengineering, Cell reprogramming, iPSC, PEG hydrogels, Synthetic microenvironment, macrogol, animal cell, bioengineering, biophysics, cell culture, cell line, controlled study, fibroblast, human, human cell, hydrogel, immunocytochemistry, induced pluripotent stem cell, microenvironment, mouse, nonhuman, nuclear reprogramming, Taverne
Citation
Caiazzo, M, Tabata, Y & Lutolf, M P 2017, Generation of induced pluripotent stem cells in defined three-dimensional hydrogels. in 3D Cell Culture : Methods and Protocols. Methods in Molecular Biology, vol. 1612, Humana Press, pp. 65-78. https://doi.org/10.1007/978-1-4939-7021-6_5