Patient-Derived Organoids from Colorectal Cancer with Paired Liver Metastasis Reveal Tumor Heterogeneity and Predict Response to Chemotherapy
Publication date
2022-11-03
Authors
Mo, Shaobo
Tang, Peiyuan
Luo, Wenqin
Zhang, Long
Li, Yaqi
Hu, Xiang
Ma, Xiaoji
Chen, Yikuan
Bao, Yichao
He, Xingfeng
Editors
Advisors
Supervisors
Document Type
Article
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cc_by
Abstract
There is no effective method to predict chemotherapy response and postoperative prognosis of colorectal cancer liver metastasis (CRLM) patients. Patient-derived organoid (PDO) has become an important preclinical model. Herein, a living biobank with 50 CRLM organoids derived from primary tumors and paired liver metastatic lesions is successfully constructed. CRLM PDOs from the multiomics levels (histopathology, genome, transcriptome and single-cell sequencing) are comprehensively analyzed and confirmed that this organoid platform for CRLM could capture intra- and interpatient heterogeneity. The chemosensitivity data in vitro reveal the potential value of clinical application for PDOs to predict chemotherapy response (FOLFOX or FOLFIRI) and clinical prognosis of CRLM patients. Taken together, CRLM PDOs can be utilized to deliver a potential application for personalized medicine.
Keywords
chemotherapy response, colorectal cancer liver metastasis, patient-derived organoid, prognosis prediction, tumor heterogeneity, Medicine (miscellaneous), General Chemical Engineering, General Materials Science, Biochemistry, Genetics and Molecular Biology (miscellaneous), General Engineering, General Physics and Astronomy
Citation
Mo, S, Tang, P, Luo, W, Zhang, L, Li, Y, Hu, X, Ma, X, Chen, Y, Bao, Y, He, X, Fu, G, Xu, X, Rao, X, Li, X, Guan, R, Chen, S, Deng, Y, Lv, T, Mu, P, Zheng, Q, Wang, S, Liu, F, Li, Y, Sheng, W, Huang, D, Hu, C, Gao, J, Zhang, Z, Cai, S, Clevers, H, Peng, J & Hua, G 2022, 'Patient-Derived Organoids from Colorectal Cancer with Paired Liver Metastasis Reveal Tumor Heterogeneity and Predict Response to Chemotherapy', Advanced Science, vol. 9, no. 31, 2204097, pp. 1-16. https://doi.org/10.1002/advs.202204097