Establishment and Culture of Human Intestinal Organoids Derived from Adult Stem Cells
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Publication date
2020-09-01
Authors
Pleguezuelos-Manzano, Cayetano
Puschhof, Jens
van den Brink, Stieneke
Geurts, Veerle
Beumer, Joep
Clevers, Hans
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Document Type
Article
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Abstract
Human intestinal organoids derived from adult stem cells are miniature ex vivo versions of the human intestinal epithelium. Intestinal organoids are useful tools for the study of intestinal physiology as well as many disease conditions. These organoids present numerous advantages compared to immortalized cell lines, but working with them requires dedicated techniques. The protocols described in this article provide a basic guide to establishment and maintenance of human intestinal organoids derived from small intestine and colon biopsies. Additionally, this article provides an overview of several downstream applications of human intestinal organoids.
Keywords
adult stem cells, human intestinal organoids, organoid cryopreservation, organoid culture establishment, organoid differentiation, organoid immunofluorescence, organoid passage, single-cell clonal organoid culture, specialized organoid reagents, Immunology
Citation
Pleguezuelos-Manzano, C, Puschhof, J, van den Brink, S, Geurts, V, Beumer, J & Clevers, H 2020, 'Establishment and Culture of Human Intestinal Organoids Derived from Adult Stem Cells', Current protocols in immunology, vol. 130, no. 1, e106. https://doi.org/10.1002/cpim.106