Cleavage inhibition of the murine coronavirus spike protein by a furin-like enzyme affects cell-cell but not virus-cell fusion
Publication date
2004-06
Authors
de Haan, Cornelis A M
Stadler, Konrad
Godeke, Gert-Jan
Bosch, Berend Jan
Rottier, Peter J M
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Document Type
Article
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Abstract
Cleavage of the mouse hepatitis coronavirus strain A59 spike protein was blocked in a concentration-dependent manner by a peptide furin inhibitor, indicating that furin or a furin-like enzyme is responsible for this process. While cell-cell fusion was clearly affected by preventing spike protein cleavage, virus-cell fusion was not, indicating that these events have different requirements.
Keywords
Endocytosis, Furin, Hydrogen-Ion Concentration, Membrane Fusion, Membrane Glycoproteins, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins, Coronacrisis-Taverne, SDG 3 - Good Health and Well-being
Citation
de Haan, C A M, Stadler, K, Godeke, G-J, Bosch, B J & Rottier, P J M 2004, 'Cleavage inhibition of the murine coronavirus spike protein by a furin-like enzyme affects cell-cell but not virus-cell fusion', Journal of Virology, vol. 78, no. 11, pp. 6048-54. https://doi.org/10.1128/JVI.78.11.6048-6054.2004