Studies on protein synthesis by protoplasts of Saccharomyces carlsbergensis II. Reversal of the RNase effect of protein synthesis by polymethacrylic acid

Abstract

The ribonuclease inhibited protein synthesis and respiration of yeast protoplasts can be restored by the addition of several polyanionic compounds, among which polymethacrylic acid proved to be the most effective one.

The results of preliminary experiments with the ultracentrifuge indicate a strong complex formation between ribonuclease and polymethacrylic acid under the applied experimental conditions. It is suggested that the reversal of ribonuclease action by polymethacrylic acid in vivo may be attributed to such complex formation between ribonuclease and polymethacrylic acid.