An LC-MS/MS method for quantification of the active abiraterone metabolite Δ(4)-abiraterone (D4A) in human plasma
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Publication date
2017-11-15
Authors
van Nuland, Merel
Rosing, Hilde
de Vries, Jelle
Ovaa, Huib
Schellens, Jan H M
Beijnen, Jos H
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Article
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taverne
Abstract
Δ(4)-Abiraterone (D4A) is a recently discovered active metabolite of the oral anti-androgen drug abiraterone acetate. For quantification of this metabolite in human plasma, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated. Human plasma samples of patients treated with abiraterone acetate were prepared by protein precipitation with acetonitrile. The method was validated over a linear range of 0.2-20ng/mL. Intra-assay and inter-assay variabilities were within ±15% of the nominal concentrations for quality control (QC) samples at medium and high concentrations and within ±20% at the lower limit of quantification (LLOQ), respectively. The described method for quantification of D4A was validated successfully and implemented to support therapeutic drug monitoring in patients treated with abiraterone acetate.
Keywords
Androstenes, Chromatography, Liquid, Drug Monitoring, Humans, Linear Models, Reproducibility of Results, Sensitivity and Specificity, Tandem Mass Spectrometry, Taverne
Citation
van Nuland, M, Rosing, H, de Vries, J, Ovaa, H, Schellens, J H M & Beijnen, J H 2017, 'An LC-MS/MS method for quantification of the active abiraterone metabolite Δ(4)-abiraterone (D4A) in human plasma', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 1068-1069, pp. 119-124. https://doi.org/10.1016/j.jchromb.2017.10.033