Electrophoretic comparison of nuclear and nucleolar proteins II. Rat pancreas

Abstract

The nuclei and nucleoli from rat pancreas were isolated and extracted successively with 0.14 M NaCl, 1 M NaCl, and 0.1 N NaOH. In the 0.14 M NaCl and 0.1 N NaOH extracts agar electrophoresis revealed differences between the nucleolar proteins and those from the non-nucleolar part of the nucleus. These findings confirm those obtained from beef pancreas. The apparatuses used for liberation and isolation of the nucleoli from very small amoubts of nuclei and for their extraction are described.