A Plasma Creatinine- and Urea-Based Equation to Estimate Glomerular Filtration Rate in Rats
Publication date
2021-03
Authors
Besseling, Paul J
Pieters, Tobias
Nguyen, Isabel T N
de Bree, Petra M
Willekes, Nel
Dijk, Adele H
Bovée, Dominique M
Hoorn, Ewout J
Rookmaaker, Maarten B
Gerritsen, Karin
Editors
Advisors
Supervisors
Document Type
Article
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taverne
Abstract
Monitoring renal function is a vital part of kidney research involving rats. The laborious measurement of glomerular filtration rate (GFR) with administration of exogenous filtration markers does not easily allow serial measurements. Using an in-house database of inulin clearances, we developed and validated a plasma creatinine- and plasma urea-based equation to estimate GFR in a large cohort of male rats [development cohort n = 325, R 2 = 0.816, percentage of predictions that fell within 30% of the true value (P30) = 76%] that had high accuracy in the validation cohort ( n = 116 rats, R 2 = 0.935, P30 = 79%). The equation was less accurate in rats with nonsteady-state creatinine, in which the equation should therefore not be used. In conclusion, applying this equation facilitates easy and repeatable estimates of GFR in rats. NEW & NOTEWORTHY This is the first equation, that we know of, which estimates glomerular filtration rate in rats based on a single measurement of body weight, plasma creatinine, and plasma urea.
Keywords
Creatinine, Estimated glomerular filtration rate, Inulin clearance, Rats, Urea, Taverne, Urology, Physiology, Journal Article
Citation
Besseling, P J, Pieters, T T, Nguyen, I T N, de Bree, P M, Willekes, N, Dijk, A H, Bovée, D M, Hoorn, E J, Rookmaaker, M B, Gerritsen, K G, Verhaar, M C, Gremmels, H & Joles, J A 2021, 'A Plasma Creatinine- and Urea-Based Equation to Estimate Glomerular Filtration Rate in Rats', American journal of physiology. Renal physiology, vol. 320, no. 3, pp. F518-F524. https://doi.org/10.1152/ajprenal.00656.2020